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EMBO Practical Course on CryoElectron Microscopy and 3D Image Processing


The Biology Department at IISER Pune is organising an EMBO practical course on cryoelecton microscopy. 

CryoElectron Microscopy and 3D Image Processing (CEM3DIP) of Macromolecular Assemblies and Cellular Tomography

Dates: December 04-16, 2022

Venue: IISER Pune

About the course:

Each passing year unveils new and path-breaking developments in various aspects of cryo-EM, which needs to reach the user community. The practical course features not only intensive training for the participants, but also includes research talks, discussions and brain-storming sessions on the use of cryo-EM to address difficult biological questions, which will help the participants address their research questions. The course will be an essential component of the various activities occurring periodically in capacity building and future developments in cryo-EM.
The EMBO practical course on Cryo-EM image processing have been organized in India since 2018 (alternate years), which has introduced and provided training to PhD students and postdoctoral fellows from all over the globe on the emerging areas of cryo-EM that has revolutionized structural biology.
Website link for the course:
  • Gayathri Pananghat, IISER Pune
  • Saikrishnan Kayarat, IISER Pune
  • Radha Chauhan, NCCS, Pune
  • Janesh Kumar, NCCS, Pune
  • Kiran Kulkarni, CSIR-NCL, Pune
  • Ramanathan Natesh, IISER Thiruvananthapuram
Course Objectives

i) To impart training in state-of-the-art techniques for structure determination using cryoelectron microscopy.

ii) Introduction to the best sample preparation and data collection strategies  (including hands-on experience of sample preparation and demonstration of remote data collection).

iii) Training in basic principles of structure determination using cryoEM, including single particle analysis, helical reconstruction and tomography applications.

iv) Hands-on practical training in the latest software suites for image processing of electron micrographs for high resolution structure determination and tomography application.

v) Exposure to the latest scientific advances in the field through research talks from eminent researchers from across the globe.

vi) Developing problem solving skills through brainstorming sessions, panel discussions and independent practice sessions on participant's data in the presence of experts in the field.

vii) Networking with experts, software developers, and researchers for gaining exposure to the global status of the research in cryoEM and learn of not only the latest but also upcoming developments.