A microscopy image showing layers in different colors and a network of neurons

Department of
Biology

Photo of Kalika  Prasad

Kalika Prasad

Associate Professor

Biology

Stem cell, regeneration and patterning in plants

+91-20-25908639

kalika.prasad@iiserpune.ac.in

Kalika Prasad obtained his PhD from Indian Institute of Science, Bangalore. He was EMBO post doctoral fellow at Utrecht University, The Netherlands and then moved to IISER Thiruvananthapuram as group leader.

Research

Cellular reprogramming and developmental plasticity in plants

The extreme fantasies of regeneration paraded by many mythological and comic characters are encompassed in one single life form: the plant. Remarkable ability to repair the damaged body parts and to regenerate organs lost in injury is prevalent across the kingdoms. While in animals this ability is restricted to specific lineages, it is universal in plants. Plants not only regrow lost parts, but even regenerate an entire new plant from a few pre-existing cells irrespective of their origin and developmental context. In response to the damage caused by biotic and abiotic factors, cells in the vicinity of wound must respond promptly and get reprogrammed to repair the damage. This is strikingly different from animal kingdom, where specialized cells can be recruited to the wound site. Plants are likely to deploy kingdom-specific mechanisms to deal with intrinsic differences such as lack of cell migration, a key cellular process, extensively utilized in animal regeneration. This makes plant regeneration an interesting model to underpin the basic principles and dynamics of self-organization.

Our laboratory uses tissue-culture-mediated de novo shoot regeneration and innate ability of restoration of tissue or organ lost in injury as models to address fundamental questions related to acquisition of pluripotent state, reconstitution of stem cells, dynamics of cellular behaviour, assembly of regulatory interactions and gene regulatory network. Most of our inferences are based on live imaging of cytological events during regeneration. We use interdisciplinary approaches which combine molecular biological tools, genetics, genomics, bio-imaging and computational modelling to unearth the mechanisms of cellular reprogramming and developmental plasticity in plant.

Selected Publications

Shanmukhan AP, Mathew MM, Aiyaz M, Varaparambathu V, Kareem A, Radhakrishnan D, Prasad K*, (2021) Regulation of touch-stimulated de novo root regeneration from Arabidopsis leaves, Plant Physiology, 187, 52-58 (Selected as Plant Physiology Article of the Week by American Society of Plant Biologists (ASPB)

Mathew MM, Prasad K*. (2021) Model systems for regeneration: Arabidopsis. Development. 148 (6), dev195347

Radhakrishnan, D., Shanmukhan, AP., Kareem, A., Aiyaz, M., Varapparambathu, V., Toms, A., Kerstens, M., Valsakumar, D., Landge, AN., Shaji, A., Mathew, MK., Sawchuk MG., Scarpella, E., Krizek, BA., Efroni, I., Mähönen, AP., Willemsen, V., Scheres, B., Prasad K*. (2020) A coherent feed forward loop drives vascular regeneration in damaged aerial organs growing in normal developmentalcontext. Development. 147: dev185710. (Selected for the highlights of the issue and featured on cover)

Durgaprasad, K., Roy, MV., Venugopal, MA., Kareem, A., Raj K., Willemsen, V., Mähönen, AP., Scheres, B., Prasad, K*. (2019) Gradient expression of transcription factor Imposes a boundary on organ regeneration potential in plants.
Cell Reports. 29(2): 453-463.e3. doi:10.1016/j.celrep.2019.08.099. (Featured on Cover, Recommended by F1000 Prime).

Radhakrishnan, D., Kareem, A., Durgaprasad, K., Sreeraj, E., Sugimoto, K., Prasad, K*. (2018). Shoot regeneration:a journey from acquisition of competence to completion. Current Opinion in Plant Biology. 41, 23-31. doi: 10.1016/j.pbi.2017.08.001.

Santuari, L., Sanchez-Perez, G.F., Luijten, M., Rutjens, B., Terpstra, I., Berke, L., Gorte, M., Prasad, K., Bao, J.L., Timmermans-Hereijgers, K., Maeo, K., Nakamura, A., Shimotohno, A., Pencik, O., Novak, K., Ljung, S., van Heesch, E., de Bruijn, E., Cuppen, V., Willemsen, A.P., Mähönen, W., Lukowitz, B., Snel, BD., de Ridder, B., Scheres, R., Heidstra. (2016). The PLETHORA Gene Regulatory Network Guides Growth and Cell Differentiation in Arabidopsis Roots. Plant Cell. 28(12), 2937-2951. (Featured on cover)

Kareem, A., Durgaprasad, K., Sugimoto. K., Du, Y., Pulianmackal, AJ., Trivedi, ZB., Abhayadev, PV., Pinon, V., Meyerowitz, EM., Scheres, B., Prasad, K*. (2015) PLETHORA genes control regeneration by a two-step mechanism. Current Biology. 25, 1017-1030. (Featured on Cover)

Mähönen, AP., Tusscher, KT., Siligato, R., Smetana, O., Díaz-Triviño, S., Salojärvi, J., Wachsman, G., Prasad, K., Heidstra, R., Scheres, B. (2014) PLETHORA gradient formation mechanism separates auxin responses. Nature. 515, 125-129